The following railroads operate in the U.S. state of West Virginia.
Common freight carriers
- Appalachian and Ohio Railroad (AO)
- Beech Mountain Railroad (BEEM)
- CSX Transportation (CSXT)
- Durbin and Greenbrier Valley Railroad (DGVR) operates Durbin Railroad and West Virginia Central Railroad (WVC)
- Elk River Railroad (ELKR)
- Kanawha River Railroad (KNWA)
- Norfolk Southern Railway (NS)
- R.J. Corman Railroad/West Virginia Line (RJCV)
- South Branch Valley Railroad (SBVR)
- Wheeling and Lake Erie Railway (WE)
- Winchester and Western Railroad (WW)
Private freight carriers
- Belpre Industrial Parkersburg Railroad (BIP)
- Kanawha River Terminal Railroad (KRT)
- Little Kanawha River Rail, Inc. (LKRR)
- Short Line Services operates Big Eagle Rail, LLC, lessor of Kanawha Rail Corporation
Passenger carriers
- Amtrak (AMTK)
- Cass Scenic Railroad
- Durbin and Greenbrier Valley Railroad
- MARC
- Potomac Eagle Scenic Railroad
- South Branch Valley Railroad
Defunct railroads
- Electric
- Appalachian Power Company
- Charleston–Dunbar Traction Company
- Charleston Interurban Railroad
- City Railway
- East Liverpool Traction and Light Company
- Grafton Light and Power Company
- Kanawha Traction and Electric Company
- Lewisburg and Ronceverte Electric Railway
- Monongahela Valley Traction Company
- Morgantown and Pittsburgh Railway
- Morgantown and Wheeling Railway
- Newell Bridge and Railway Company
- Ohio Valley Electric Railway
- Pan Handle Traction Company
- Parkersburg and Ohio Valley Electric Railway
- Princeton Power Company
- South Morgantown Traction Company
- Steubenville, East Liverpool and Beaver Valley Traction Company
- Steubenville, Wellsburg and Weirton Railway
- Tyler Traction Company
- Union Traction Company
- Wellsburg, Bethany and Washington Railway
- West Virginia Traction and Electric Company
- Wheeling and Elm Grove Railway
- Wheeling Traction Company
Notes
